卵胞漿內(nèi)單精子注射技術(shù)(intracytoplasmic sperm injection, ICSI)）完全或不完全受精失敗非常罕見，但是部分患者由于精子缺乏激活卵母細(xì)胞的物質(zhì)而導(dǎo)致ICSI反復(fù)的完全或不完全受精失敗。本文報(bào)道的是一例嚴(yán)重的弱畸精子患者，出現(xiàn)了反復(fù)的ICSI不完全受精失敗。在患者的第二個(gè)ICSI周期中，10枚卵母細(xì)胞經(jīng)ICSI技術(shù)受精后，僅有一枚卵母細(xì)胞受精。遂采用鈣離子載體A23187對(duì)ICSI未受精卵母細(xì)胞進(jìn)行激活處理。未受精卵母細(xì)胞激活，獲得胚胎，移植后獲得臨床妊娠，并健康新生兒的出生。由此可見，對(duì)ICSI未受精卵母細(xì)胞實(shí)施“補(bǔ)救性卵母細(xì)胞激活技術(shù)”能挽救ICSI完全或不完全受精失敗。北京大學(xué)人民醫(yī)院計(jì)劃生育與生殖醫(yī)學(xué)科鹿群

[Abstract]

Total or near-total fertilization failure after intracytoplasmic sperm injection (ICSI) is a rare event, but it occurs repeatedly because of sperm defects in activating oocyte. The case presents a successful pregnancy and live birth after calcium ionophore A23187 (A23187) activation on one-day-old unfertilized oocytes in a patient whose husband suffered oligoasthenoteratozoospermia, and who had experienced repeated near-total fertilization failure after ICSI. In the second ICSI cycle, only one oocyte was fertilized while nine were unfertilized. Oocyte activation with A23187 were performed on the one-day-old unfertilized oocytes after ICSI and resulted in fertilization and embryo transfer. A clinical pregnancy was achieved and a healthy baby was born. To our knowledge, this is the first reported case of a healthy birth after oocyte activation on the one-day-old unfertilized oocyte. This indicates that “rescue oocyte activation” on one-day-old unfertilized oocytes after ICSI may be help for preventing total or near-total fertilization failure after ICSI.

Chin Med J 2012;125(3):546-548